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RPMI Media

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RPMI Media

RPMI 1640 Medium no folic acid

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells including HeLa J...

Product Description

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications.

This RPMI is modified as follows:

With Without
• L-glutamine • HEPES
• Phenol Red • folic acid
 

The complete formulation is available.

Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Product use

For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
specifications
Form: Liquid
Glutamine: L-Glutamine
HEPES Buffer: No HEPES
Other Additives： No Folate (No Folic Acid)
Phenol Red Indicator: Phenol Red
Product Line: VictoryX
Shipping Condition: Room Temperature
Sodium Pyruvate Additive: No Sodium Pyruvate
Volume (Metric): 500 ml

Pre：1.RPMI 1640 Medium HEPES
Next：RPMI 1640 Medium no Glucose

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